FastQCFastQC Report
Fri 13 Dec 2019
HC2GYBCX3_l01_n02_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2GYBCX3_l01_n02_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57312465
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGNNNNNCNNCG15427052.6917442828536515Illumina Single End PCR Primer 1 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT15468500.050.6878478
GAGCGTC15469800.050.4631469
AAGAGCG15806700.050.034827
GAAGAGC16004850.049.4645086
TCGGAAG16043200.049.364193
CGGAAGA16102950.049.25744
GGAAGAG16155950.049.0987745
ATCGGAA16189700.048.7547042
GATCGGA16332400.048.327011
TTAAAAA9384300.020.62793255-59
ATTAAAA9236300.020.53357955-59
TAAAAAA7418300.019.10614655-59
AAAAAAA33120800.018.95604560-64
AAAAAAC2275300.018.47811760-64
CGTAAGA92300.017.56643190-94
TCGTAAG90250.017.50139490-94
TCATTAA8871700.017.05386250-54
ATCATTA8935550.016.9321250-54
CATTAAA9067600.016.82383750-54
TATCATT8817100.016.79461150-54
Produced by FastQC (version 0.11.5)