FastQCFastQC Report
Fri 13 Dec 2019
HC2GYBCX3_l01_n02_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2GYBCX3_l01_n02_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35954965
Sequences flagged as poor quality0
Sequence length151
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGNNNNNCNNCG5102111.4190279423161725Illumina Single End PCR Primer 1 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC3389500.077.301519
GATCGGA3427450.077.192271
AGAGCGT3423750.076.9316948
TCGGAAG3461700.076.724943
AAGAGCG3471100.076.5654757
CGGAAGA3486750.076.4324954
ATCGGAA3479600.076.0560152
GAAGAGC3507000.075.902376
GGAAGAG3584200.074.413435
GAGCGGC21750.051.3972479
AGCGAGC9150.049.410557140-144
TGCGAGC8450.044.58645140-144
CGCGAGA5154.9112714E-1043.89385140-144
AGAGCGG27450.043.778948
GCTCGGA16850.043.5972441
TCGCTCG12850.041.04729140-144
AGAGCGC30250.037.3509948
GAGCGCC25400.036.780979
GGTCGGA15850.035.5165631
ACGCACG17450.034.544918140-144
Produced by FastQC (version 0.11.5)