FastQCFastQC Report
Fri 13 Dec 2019
HC2GYBCX3_l01_n02_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2GYBCX3_l01_n02_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46061300
Sequences flagged as poor quality0
Sequence length151
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG315650.039.5140383
ATCGGAA330500.037.7144172
GATCGGA343600.036.2649041
AGAGCGT342400.036.2169848
AAGAGCG395250.032.011097
CGGAAGA402400.031.3628834
GGAAGAG432050.029.4417325
GAGCGTC454950.027.3450839
GAAGAGC492000.025.8705086
CTAGTAC23900.021.830023140-144
TAGCCTA28000.020.408102140-144
TAGCTAG29400.020.281343140-144
TAGACTA22650.019.744091140-144
GCTAGTA28150.019.416775140-144
CTAGGTA28800.018.97855140-144
TATCTAG32800.018.936466140-144
CTATCTA47950.018.652914140-144
CATACTA36900.018.179008140-144
TAGTGTA31950.017.885035140-144
TACTCTA35750.017.37388140-144
Produced by FastQC (version 0.11.5)