FastQCFastQC Report
Fri 13 Dec 2019
HC2GYBCX3_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2GYBCX3_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10515401
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATCATCTCGTATGCC204940.19489508769090214TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC147500.14027044712797923TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCATCTCGTATGCC143400.13637140419086252TruSeq Adapter, Index 14 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATCATCTCGTATGCC134420.1278315491724947TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAACCAGATCATCTCGTATG128750.12243945808628696TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGATCATCTCGTATG115530.10986742207929114TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC114370.10876427822391177TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC570900.064.973738
TCGGAAG620650.061.5091483
GATCGGA640400.060.8753551
ATCGGAA638200.060.85322
CGGAAGA631300.060.0597654
GAGCACA644450.057.293579
AAGAGCA667950.056.2091757
GAAGAGC704650.053.3624236
GGAAGAG708950.053.0853735
CTAGCAA8500.030.893986145
AGTAGGG8200.030.338766145
TAGTATG11900.030.197126145
GGTGACT15600.029.6618541
GTGACTG16200.029.5755022
TGACTGG16750.028.6043663
ACTGGAG17350.027.614385
CGAGTTA4551.524937E-624.300648145
TAGCGTG4503.8431885E-521.499323145
GGAGTTC20400.021.3188488
CAGTCTT15050.021.121782145
Produced by FastQC (version 0.11.5)