FastQCFastQC Report
Fri 13 Dec 2019
HC2GYBCX3_l01_n01_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2GYBCX3_l01_n01_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57312465
Sequences flagged as poor quality0
Sequence length151
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC842691214.70345412642782TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCACCTCGTATGC1727240.301372485025727TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAACTCGTATGC1256140.21917396154571261TruSeq Adapter, Index 7 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG1065780.18595954649656057TruSeq Adapter, Index 7 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAGCTCGTATGC682190.11902995273366797TruSeq Adapter, Index 7 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTATG113950.069.49873145
GAGCACA20495500.053.7031149
AGAGCAC20672950.053.38498
ATACGTC157750.053.124825145
AAGAGCA20957550.052.781257
GAAGAGC21171000.052.287776
TCGGAAG21334150.052.035743
CGGAAGA21298150.051.9422074
GGAAGAG21276550.051.8643465
ATCGGAA21744100.051.350192
GATCGGA21959800.050.877971
CGCGTAT161950.046.555634145
GTACACG181950.046.357147145
CGAGTCA154000.046.317318145
TATGAAC217550.040.51657145
CTCACGT204650.039.757393145
ATAGCAC201150.037.078407145
ATATCGG220800.034.147125145
TGTCGTA200000.033.22343145
GACTGAA253550.032.838486145
Produced by FastQC (version 0.11.5)