FastQCFastQC Report
Fri 13 Dec 2019
HC2GYBCX3_l01_n01_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2GYBCX3_l01_n01_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35954965
Sequences flagged as poor quality0
Sequence length151
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC27657267.692194944425617TruSeq Adapter, Index 5 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGT1141840.3175750553504919TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGACCTCGTATGC632120.1758088208401816TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAACTCGTATGC491970.13682950324106838TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3602200.0104.7717061
TCGGAAG3626150.0103.7290343
ATCGGAA3677550.0102.664742
CGGAAGA3773850.099.333754
AGAGCAC3755350.099.258188
GAAGAGC3806700.098.257226
GAGCACA3787500.098.1627359
GGAAGAG3855250.096.842525
AAGAGCA3943450.094.803467
TACGGCG24750.076.750256
CGGCGAC33050.057.624718
ACGGCGA33600.056.5147367
GGCGACC38100.049.9867869
ATACGGC40900.046.5812265
GCTCGGA12150.042.287235145
GATCGGG14150.041.5901871
GATACGG53550.035.891364
CGGAAGG44000.031.2010154
GAGGCAC40250.027.8333459
CGGAAAG44000.026.4890254
Produced by FastQC (version 0.11.5)