FastQCFastQC Report
Fri 13 Dec 2019
HC2GYBCX3_l01_n01_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2GYBCX3_l01_n01_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46061300
Sequences flagged as poor quality0
Sequence length151
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1196100.25967569304383503TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG427600.042.2360463
ATCGGAA455650.040.0296332
GATCGGA481350.037.982691
AGAGCAC478750.037.4282958
GAGCACA519800.034.526389
CGGAAGA563700.032.0882724
AAGAGCA571700.031.5488227
GGAAGAG596500.030.3708045
GAAGAGC691450.026.2813476
CTAGGCC55100.016.882645145
CTAGCGC73400.014.142873145
CTAGGGC42200.014.056694145
CTAGGCG56500.013.600953145
CTCGTAT278450.012.69330940-44
TCGTATG295550.012.23568740-44
GTATCTC257850.012.1341335-39
CTAGCAG72350.011.739374145
CTAGATG43500.011.467148145
CTAGCAC47300.011.40097145
TATCTCG279150.011.19621635-39
Produced by FastQC (version 0.11.5)