FastQCFastQC Report
Wed 21 Mar 2018
HC2CWBCX2_l02n03.333000000cc0aa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2CWBCX2_l02n03.333000000cc0aa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129120309
Sequences flagged as poor quality0
Sequence length33
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTACTCTGCGTTGATACCACTGCTTCCGCG5108200.395615534036555No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4480680.3470158981729203No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCGCG3713150.2875728867718246No Hit
TACGTACTCTGCGTTGATACCACTGCTTCCGCG2626110.20338473632370258No Hit
GTACTCTGCGTTGATACCACTGCTTCCGCGGAC2567080.19881303103139258No Hit
AACGTACTCTGCGTTGATACCACTGCTTCCGCG2349630.1819721481614484No Hit
GCGTTACTCACTAAAAAAAAAAAAAAAAAAAAA1953430.1512875871447922No Hit
GTACGTACTCTGCGTTGATACCACTGCTTCCGC1826700.14147270976558768No Hit
GGCCAGCGTTACTCACTAAAAAAAAAAAAAAAA1441770.11166097813474099No Hit
GTGAATGGGCCAGCGTTACTCACTAAAAAAAAA1417360.10977049319174105No Hit
CGTACTCTGCGTTGATACCACTGCTTCCGCGGA1390160.10766393069892669No Hit
TACTCTGCGTTGATACCACTGCTTCCGCGGACA1342720.10398983788057693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA486750.018.362181
GTATCAA1612950.018.0858691
TTCCGCG2297000.017.47181527
CTTCCGC2768750.014.49837526
CGCGGAC535100.013.77941127
GACCTAG159750.012.15895811
GCGGACA351000.011.46346527
CCTAGTA175700.011.22563813
TGACCTA183100.011.14640410
CTAAGAC349450.010.9898673
TAAGACA383700.010.7129854
TATCAAC2688850.010.63423252
CCGCCCG278900.010.30394611
ACCTAGT190950.010.26436312
GCTTCCG3946300.010.15662925
GTGACCT206200.010.1460429
CCGCGGA729700.010.13581726
GAGTACG669000.010.008351
ATCAACG2830100.010.0024163
CGGACAG173300.09.99955427
Produced by FastQC (version 0.11.2)