FastQCFastQC Report
Wed 21 Mar 2018
HC2CWBCX2_l02n02.332000000cc0ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2CWBCX2_l02n02.332000000cc0ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129120309
Sequences flagged as poor quality0
Sequence length9
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NGNCNNCTA2970164823.00308001896123No Hit
NGNCANCTA61516104.764246653096222No Hit
NGNCTNCTA39466903.05659894292849No Hit
NGNCGNCTA33203832.5715420182273574No Hit
AGNCNNCTA18571111.438279550585648No Hit
NGNCCNCTA8906800.6898062798161364No Hit
NNNNNNNNN7603730.5888872214517392No Hit
NGNCNNCTC3197140.247609382657224No Hit
GGNCANCTA2976320.23050750289019215No Hit
GGNCTNCTA2040730.15804872338092066No Hit
AGNCANCTA1974280.152902360232115No Hit
GGNCGNCTA1874420.14516848778606933No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)