FastQCFastQC Report
Wed 21 Mar 2018
HC2CWBCX2_l02n01.331000000cc0a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2CWBCX2_l02n01.331000000cc0a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129120309
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTCCGAGCC5405980.41867774650384393No Hit
GCTGTCTCTTATACACATCTCCGAGCC3864060.29926043624942067No Hit
ACTGTCTCTTATACACATCTCCGAGCC2734780.21180091816539875No Hit
GTCTCTTATACACATCTCCGAGCCCAC2596300.2010760367681586No Hit
TCTGTCTCTTATACACATCTCCGAGCC2445700.18941249590720852No Hit
ACCTGTCTCTTATACACATCTCCGAGC1916310.1484127489193044No Hit
CTCTTATACACATCTCCGAGCCCACGA1840750.14256084222970686No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA1475420.11426707474809404No Hit
TGTCTCTTATACACATCTCCGAGCCCA1416600.109711633357383No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTTTT11697900.019.8892319
ATTTTTT21975400.019.88449920
GGATTTT6740600.019.686318
GCTTTTT13039150.019.66427819
CTTTTTT25757300.019.6405820
GGCTTTT7124900.019.53200518
TGTTTTT10626400.019.50737419
GTTTTTT48309000.019.48966620
TGATTTT2368300.019.46954718
GGGCTTT3872450.019.45410217
AGTTTTT4374150.019.36277619
GTGTTTT5402600.019.31474318
CGTTTTT5473800.019.30980919
GGGATTT3797050.019.27779217
TATTTTT4785100.019.22192219
TGGTTTT6547200.019.15966618
GGTTTTT28726850.019.15394219
TGCTTTT2832050.019.1401118
CGGTTTT3682050.019.11205118
GAGTTTT2375550.019.03189318
Produced by FastQC (version 0.11.2)