FastQCFastQC Report
Wed 21 Mar 2018
HC2CWBCX2_l01n03.333000000cc09d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2CWBCX2_l01n03.333000000cc09d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123790160
Sequences flagged as poor quality0
Sequence length33
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGTACTCTGCGTTGATACCACTGCTTCCGCG4988330.4029666008994576No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4043910.32667459190617415No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCGCG3611890.2917752105660094No Hit
TACGTACTCTGCGTTGATACCACTGCTTCCGCG2556610.2065277240129587No Hit
GTACTCTGCGTTGATACCACTGCTTCCGCGGAC2523420.20384657391185212No Hit
AACGTACTCTGCGTTGATACCACTGCTTCCGCG2303100.18604871340339169No Hit
GCGTTACTCACTAAAAAAAAAAAAAAAAAAAAA1904750.15386925745955898No Hit
GTACGTACTCTGCGTTGATACCACTGCTTCCGC1786240.14429579863213682No Hit
GGCCAGCGTTACTCACTAAAAAAAAAAAAAAAA1408530.11378368038299652No Hit
GTGAATGGGCCAGCGTTACTCACTAAAAAAAAA1367200.11044496590035913No Hit
CGTACTCTGCGTTGATACCACTGCTTCCGCGGA1349020.10897635159369695No Hit
TACTCTGCGTTGATACCACTGCTTCCGCGGACA1331380.10755135949416335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA473250.018.3591561
GTATCAA1557700.018.0956611
TTCCGCG2210050.017.4235427
CTTCCGC2669750.014.43312826
CGCGGAC516300.013.475059527
GACCTAG152750.011.84120811
GCGGACA343600.011.48598827
CTAAGAC330050.010.8062533
CCTAGTA170650.010.79784813
TGACCTA177750.010.66161610
TATCAAC2599200.010.6330182
TAAGACA362200.010.6261664
CCGCCCG275650.010.17559911
GTGACCT191500.010.1285819
ACCTAGT181200.010.1167912
GCTTCCG3829200.010.06847225
CCGCGGA697850.010.025290526
ATCAACG2735200.010.0069283
TATACTG169000.09.9498375
CGCCCGC288900.09.9478512
Produced by FastQC (version 0.11.2)