FastQCFastQC Report
Wed 21 Mar 2018
HC2CWBCX2_l01n02.332000000cc090.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2CWBCX2_l01n02.332000000cc090.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123790160
Sequences flagged as poor quality0
Sequence length9
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NGNCNNCNA76613236.188959607128709No Hit
NGNCNNCTA72841885.8843029203613595No Hit
NGNCANCTA21713981.7540958021219133No Hit
NGNCNNCGA18726421.5127551333643967No Hit
NGNCTNCTA16809921.3579366889904658No Hit
NGNCGNCTA11543960.9325426188963646No Hit
NGNCNNCAA8931910.7215363482848718No Hit
NGNCANCNA8336570.6734436727442634No Hit
NGNCNACTA6188310.4999032233256666No Hit
NNNNNNNNN5384680.4349844931131845No Hit
NGNCGNCNA4825730.389831469641852No Hit
NGNCTNCNA4705480.3801174503692377No Hit
NGNCNCCTA4160900.3361252622987158No Hit
NGNCNNCCA3564190.2879219156029849No Hit
NGNCCNCTA3183940.25720461141661016No Hit
NGNCNGCTA2978650.2406209023398952No Hit
NGNCCNCNA1600360.12928006555609914No Hit
NGNCANCGA1448050.11697617968988812No Hit
NGNCNTCTA1338110.10809502144596952No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)