FastQCFastQC Report
Wed 21 Mar 2018
HC2CWBCX2_l01n01.331000000cc093.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC2CWBCX2_l01n01.331000000cc093.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123790160
Sequences flagged as poor quality0
Sequence length27
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTCCGAGCC5301200.42824082301856625No Hit
GCTGTCTCTTATACACATCTCCGAGCC3773670.30484410069427165No Hit
ACTGTCTCTTATACACATCTCCGAGCC2668640.2155777163548379No Hit
GTCTCTTATACACATCTCCGAGCCCAC2567970.20744540599995995No Hit
TCTGTCTCTTATACACATCTCCGAGCC2407310.194466991560557No Hit
ACCTGTCTCTTATACACATCTCCGAGC1875390.1514975019016051No Hit
CTCTTATACACATCTCCGAGCCCACGA1825300.1474511382811041No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAA1422190.11488716065961949No Hit
TGTCTCTTATACACATCTCCGAGCCCA1381090.11156702600594426No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTTTT11239150.019.90266819
ATTTTTT21084900.019.86932820
GGATTTT6463950.019.68872818
GCTTTTT12489600.019.65488419
CTTTTTT24698700.019.62031220
TGTTTTT10200250.019.53385419
GGCTTTT6800150.019.50349818
GTTTTTT46390250.019.4756920
TGATTTT2304850.019.47430618
GGGCTTT3706100.019.40398417
AGTTTTT4192850.019.34569719
GTGTTTT5193450.019.339518
TATTTTT4554500.019.3059719
CGTTTTT5240300.019.27907219
GGGATTT3646150.019.26014717
GGTTTTT27608550.019.1360419
TGGTTTT6280450.019.13230518
CGGTTTT3503700.019.11698218
TGCTTTT2727350.019.10433618
TCTTTTT6087800.019.0641719
Produced by FastQC (version 0.11.2)