FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_b3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_b3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15205977
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC20700.025.32956715
GCGGGCG21600.024.43378614
TCGGGCG23300.024.1330327
GGCGGGC22450.023.66248313
ATCGGGC26700.021.3223846
GGGCGGC27300.020.0909489
TAGCCCG28500.018.88208634
GCACACG34950.018.35760311
CGGGCAT21400.018.2188931
CGTATGC22750.017.89202144
TATGCCG24500.016.75525746
AATCGGG38700.016.5830735
ACGTCTG38150.016.54668815
CACTCGG43900.016.10882631
CGCGCCT22550.016.0657161
CGGGCGG37300.015.8147838
GGCGCAT34250.015.71168617
ACACGTC41100.015.694797513
CACACGT42250.015.4307912
ACTCGGC38750.015.31202732
Produced by FastQC (version 0.11.2)