FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_b3_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_b3_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16711367
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC25550.026.59717444
TATGCCG26050.025.69033446
GCCAGTA54300.023.1925951
GTGTGCG44400.022.8423828
GTATGCC31150.022.3698945
GCACACG34650.022.30020311
ACGTCTG34850.021.47921615
TCGTATG33100.020.00924943
GTGCGAG51100.019.84691810
ATGCCGT33300.019.78649147
CGGGCGC40400.018.61386515
CGCGTAT23300.018.362091
GGCGGGC41150.018.27477513
CACACGT40350.018.20964212
TGCGAGT56300.018.01370211
TAGCCCG42650.017.63236634
TGTGTGC61550.017.5427847
AGTAGTC70300.017.1776544
ACCCGTA59900.017.04681432
CGCTATG57300.016.9171629
Produced by FastQC (version 0.11.2)