FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_b2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_b2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13370645
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG31650.025.8316218
ACGTCTG32100.025.14634315
GCACACG31750.024.98923711
ATCGGGG12900.021.92777840
ACTCCCG36600.021.86681632
CACACGT37350.021.70431712
GTGCGAG38650.021.33121910
GCCAGTA45300.020.9454381
TGCGAGT41850.019.94751511
ACCCGTA41550.019.59384232
CACGTCT41650.019.3803514
TGTGTGC43850.019.2740737
CGCGTAT15500.019.1435171
CCCGAAT40600.019.1173235
TGTGCGA44500.018.9145269
CCCGTAA44750.018.19278533
CTGACAT56900.018.191311
GCGAGTC45700.018.11606812
CGGGGTG7700.017.92040851
CGGGCGC22800.017.85299915
Produced by FastQC (version 0.11.2)