FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_b2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_b2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12649536
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC21300.028.34101344
TATGCCG21350.027.95205346
GCACACG27000.023.63531711
GTATGCC25700.023.62326445
GTGTGCG33450.021.9657258
ACGTCTG29450.021.55217715
ATGCCGT27950.021.35148447
TCGTATG28900.021.12681843
GCCAGTA43800.020.5610471
CACACGT33100.019.27948812
GTGCGAG38850.018.82370810
CGCGTAT16450.018.668221
CTCGTAT32700.018.35517142
CGGGCGC29800.016.90033115
CACGTCT37850.016.8600714
GGCGGGC30200.016.67628913
TGTGTGC45950.016.5909237
TGCGAGT43500.016.49405911
GCCGTCT36000.016.48169149
TGTGCGA46050.016.2550669
Produced by FastQC (version 0.11.2)