FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_b2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_b2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10383074
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190480.18345241495919223No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG104560.10070235462060657RNA PCR Primer, Index 44 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC34550.044.92814644
TATGCCG34750.044.1736646
TCGTATG39950.038.8552543
GTATGCC40450.038.2906145
ATGCCGT41200.037.42557547
CACTATA43100.037.0566531
GCACACG47100.034.64130411
TATAATA46500.034.49549534
ACGTCTG47850.034.02706515
CTATAAT47700.033.9892733
TGCCGTC45250.033.77192748
TATCTCG46350.033.7138839
ACACGTC49800.032.83290513
CTCGTAT48050.032.5207842
CACACGT50350.032.4742512
ACTATAA51150.031.49442132
CACGTCT54850.029.99852814
GCCGTCT53600.028.38210149
TAATATC56850.028.03260236
AGCACAC60300.027.57337810
Produced by FastQC (version 0.11.2)