FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_b1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_b1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18116986
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGT24050.026.2547441
GCACACG61500.024.7351311
ATCGGGG22000.023.99013340
ACGTCTG64250.023.6766915
ACACGTC63850.023.60897813
TCGGGCG24600.022.8577987
CACACGT67000.022.75636912
ACTACAG70600.021.05917232
CGGGCAT27750.020.2673241
ATCGGGC29250.020.053666
CGGGCGC26500.020.04612215
CACGTCT76000.020.01603714
CACTACA74200.019.99094231
TACAGCA76450.019.76329434
CGGCAAT50700.019.3958441
GCGGGCG27450.019.3522514
GGCGGGC27900.019.16391413
CGCGCCT36850.019.0077321
GGGCGGC28150.018.9938249
CATCGGG37750.018.5500139
Produced by FastQC (version 0.11.2)