FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_b1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_b1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11937425
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27300.036.39122846
CGTATGC28050.035.4174644
ATGCCGT33150.029.76112747
TCGTATG34650.028.87062643
GTATGCC35050.028.24612845
GCACACG38500.026.43111211
CGCGCGT16000.026.30861
ACGTCTG41400.024.91252315
CACACGT43000.024.38684712
CTCGTAT40400.024.1639742
ACACGTC44550.023.22875213
CTAATCG43450.023.10318833
CGCGTAT13150.023.0895141
TCGGGCG20850.022.8320757
CACGTCT46350.022.5494614
TGCCGTC45500.021.9103748
ATCGGGC22550.021.2679756
GCCGTCT47900.021.10080349
ACTAATC47900.020.95685832
GGCGGGC22100.020.91533713
Produced by FastQC (version 0.11.2)