FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_b1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_b1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14159385
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37700.040.71851346
CGTATGC38200.040.4550444
GTATGCC44750.034.30303645
CACCTAT45550.034.15580431
TCGTATG46600.033.60673543
ATGCCGT47850.032.22509847
GCACACG50850.031.20427311
ACCTATA50250.031.1670832
ACGTCTG51400.030.93759515
TCACCTA52950.029.4465930
CTATACA53750.029.39421334
CTCGTAT53800.028.33987442
CACACGT56700.028.28886812
ACACGTC57050.027.87346313
CCTATAC57150.027.46448733
CACGTCT61800.025.95416614
TCGGGCG25700.025.6382797
CGCGCGT18650.025.3454131
TGCCGTC61600.025.03190848
GCCGTCT64250.023.99954849
Produced by FastQC (version 0.11.2)