FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_b0_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_b0_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19670752
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29950.023.61205548
CGTATGC32200.022.17635346
GCACACG43850.018.17208511
GTATGCC40050.018.00194447
ACGTCTG44600.017.7116815
TCGTATG43050.016.50680745
ATGCCGT41600.016.50203949
AGTCACG47150.016.24256528
ACACGTC51450.015.42068713
CGGGCGC16050.015.25957415
CACACGT52300.015.17010312
GTCACGT50800.014.93952529
ACGTGAT51700.014.61254632
TCACGTG52400.014.4833230
GCCAGTA51400.014.3652251
CACGTGA53900.014.14411631
GCGGGCG18250.013.79788214
CACGTCT58450.013.39657614
TCGGGCG18900.013.3245377
CTCGTAT52550.013.194180544
Produced by FastQC (version 0.11.2)