FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_b0_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_b0_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23073213
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG38550.028.72449946
CGTATGC39750.028.11708344
GCACACG52300.025.39354311
GTATGCC45950.024.54917145
ACGTCTG54600.024.07077215
GTGTGCG47350.023.3140038
AGTCACG59700.022.24680328
GCCAGTA71350.022.1965081
ATGCCGT51950.021.04972647
TCGTATG52500.021.0256943
CACACGT63450.020.82265712
GTCACGA65350.020.16490429
GTGCGAG57650.019.62648610
CACGTCT72750.018.2551114
CGCGTAT25950.018.0828741
CTCGTAT64250.017.61003942
ACCCGTA63150.017.58976232
TGCGAGT65600.017.1952411
CGGGCGC34950.017.17353615
AGCACAC79300.017.0522210
Produced by FastQC (version 0.11.2)