FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_b0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_b0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11040773
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTAG19000.028.14150631
TATGCCG17200.026.67402346
CGTATGC18050.025.8000144
GCACACG23400.024.02853211
ACCTAGC23850.022.27417232
GTGTGCG22750.021.8359458
GTATGCC22600.020.75858545
GCCAGTA33600.020.4359651
ATGCCGT23900.019.77361147
CACACGT28250.019.65933412
TCACCTA27200.019.65749230
ACGTCTG28050.019.06146415
TCGTATG24150.018.85472143
GTGCGAG27100.018.32966610
CGCGTAT13900.017.6248281
ACCCGTA29050.017.45582832
TGTGCGA30150.017.162219
GCGAGTC31350.016.17488312
CCTAGCT33600.015.91356833
TATCTCG29300.015.89400739
Produced by FastQC (version 0.11.2)