FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a6_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a6_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12104942
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG28550.019.5740878
CGCGTAT16850.017.8151971
GCCAGTA41750.017.6859591
GCACACG20950.017.61791211
GTGCGAG33900.016.48448410
ACGTCTG23950.015.98745415
TGCGAGT37900.015.01756111
CGCTATG43950.014.991631529
CACACGT25400.014.53136912
TGTGTGC40900.014.42297
CGTATGC12900.014.17243444
GGCTGCC45550.014.16169143
ACCCGTA40250.014.14157932
GCGAGTC41200.013.81475512
AGTAGTC50850.013.7742894
CGGGCGC28300.013.77380415
CGGAATA25350.013.74437534
TGTGCGA41050.013.6973989
GTCGCTA48050.013.56856327
CCCTGTG48950.013.53911763
Produced by FastQC (version 0.11.2)