FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a6_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a6_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10246159
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203980.1990794794420036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC21250.032.1410844
TATGCCG20550.031.72534246
TCGTATG26550.025.4651243
GCACACG28350.025.3081411
GTATGCC27150.025.15658445
ATGCCGT26350.024.61100447
ACGTCTG30000.023.8012115
CACACGT31500.023.21546612
CTCGTAT28500.022.87530542
TCGGGCG22450.022.4347087
CGCGTAT12050.021.1884161
CGCGCCT33800.020.9262281
CGGCAAT35100.020.8392731
CGCGCGT14350.020.6776081
ACACGTC34750.020.54790713
ATCGGGC25650.019.7739936
GGCGGGC24900.018.97913
GTCACCC37050.018.90074729
CACGTCT38450.018.8394714
GCCAGTA34600.018.5477261
Produced by FastQC (version 0.11.2)