FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a6_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a6_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11392266
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGT15850.025.251531
TATGCCG17500.022.07708446
CGCGTAT13300.021.7914751
GCACACG27700.020.42329611
TCGGGCG18400.020.0613487
CGTATGC19350.019.96653244
ACGTCTG29600.019.81113815
CGCGCCT25000.019.4597851
CGGGCGC17850.019.13150815
GGCGGGC18550.018.96792813
CACACGT30750.018.62193112
GCGGGCG18950.018.38503314
CGGGCAT22800.018.1595611
ATCGGGC22050.017.3690956
ACACGTC34150.016.86900313
CGCGGAT15100.016.6803381
GTATGCC24850.016.65805245
CGGCAAT32150.016.312541
CGCGGCT22900.016.1215671
CACGTCT36650.015.81202614
Produced by FastQC (version 0.11.2)