FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a5_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a5_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9087291
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG29450.025.30078711
ACGTCTG31800.024.51611315
CGCGCGT10750.023.7507441
CACACGT33900.022.18322812
ACACGTC35400.021.3409413
CACGTCT38500.019.80134414
CGCGTAT10100.019.130251
ATGGGGG21700.018.9170240
CATGGGG23350.018.4669839
CGCGGAT13250.018.227881
CGTCTGA44100.017.60025816
TGGGGGG17950.017.10364541
CGGGCAT15850.016.9792821
GCATGGG27300.016.30036738
AGCACAC47150.016.09566510
CAGTCAC47800.015.660892527
GGCATGG31650.015.25920337
CTGAACT52550.015.09820919
CCAGTCA51750.015.06555626
CGGGGTG6000.014.94856851
Produced by FastQC (version 0.11.2)