FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a5_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a5_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13435288
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGGCG25500.035.84956433
CGTATGC24850.027.06807144
CAGGCGA35400.026.50558534
TATGCCG25500.025.97305746
GCACACG39650.023.40219311
CACACGT41400.022.57977712
ACGTCTG41800.022.52849815
ACACGTC41800.022.4463113
ACCAGGC42100.022.20574632
GTATGCC33150.021.01962945
AGGCGAT45700.020.2292935
ATGCCGT33950.018.89884447
CACGTCT50250.018.73998514
TCGTATG35200.018.42309843
CGCGCGT10450.018.1600381
CGTCTGA52250.018.02320116
CGGGCAT21150.017.945381
CGCGGAT18900.016.6130711
CGCGTAT14450.016.237211
AGCACAC59000.015.96092610
Produced by FastQC (version 0.11.2)