FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a5_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a5_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19319755
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC30250.023.49092344
TATGCCG32250.021.60716846
GCACACG41250.021.49092111
ACGTCTG43950.019.30752415
CGGGCGC25200.019.30051615
GGCGGGC27150.018.1686313
GCGGGCG26950.017.91910414
CACACGT49750.017.47265612
GCCAGTA52400.017.2508431
GTATGCC42550.017.10610845
TCGGGCG32250.016.9013567
ATGCCGT39650.016.87857847
TCGTATG41800.016.7524843
CGGCAAT52750.015.8282611
ATCGGGC35850.015.4950836
CGCTATG41050.015.04244129
TAGCCCG34150.014.84907734
GTGTGCG28000.014.7843698
ACACGTC57900.014.59632513
CACGTCT59300.014.25142914
Produced by FastQC (version 0.11.2)