FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a4_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a4_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15889010
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC26300.027.01883344
TATGCCG30250.023.03491246
GCACACG36050.022.39066911
GTATGCC33200.021.4038145
CACGATA37250.021.20693834
ACGTCTG38600.020.37517415
CACACGT40750.019.89277312
TCGTATG35550.019.89177143
ATGCCGT36900.019.3509347
TATCTCG41750.017.76440839
GCCAGTA41200.017.3359851
CTCGTAT43300.016.72984742
GTGTGCG26450.016.5636638
CACGTCT49050.016.03423314
ACACGTC49550.015.8030213
AGCACAC55950.015.16690410
ACGATAT52450.015.0610335
CGGGCGC16550.014.5899515
CCACGAT57850.014.43057233
CGCGTAT13650.013.6501211
Produced by FastQC (version 0.11.2)