FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15055007
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG28250.021.0027798
ATCGGGG17650.020.12998640
GCACACG31600.018.99341211
CGCGTAT14050.018.173461
ACGTCTG33300.018.12760515
GTGCGAG34250.016.51717210
GCCAGTA39850.016.1918141
CACACGT38400.015.89958412
GATCGGG28300.015.35821839
CGGGCGC21100.015.04014415
TGCGAGT38900.014.45369311
ACCCGTA41400.013.91522232
TGTGCGA41400.013.8314389
CGCGGGG6500.013.8020061
GGCGGGC23100.013.588551513
GCGAGTC43750.013.48223212
TAGCCCG24750.013.3804334
TGTGTGC46700.013.2962657
CCCGTAA42650.013.2648333
CGGCAAT42100.013.1955041
Produced by FastQC (version 0.11.2)