FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a4_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a4_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14272459
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTGCG26800.025.229728
CGTATGC22000.024.616844
TATGCCG22650.023.75839646
GTGCGAG33600.020.22524310
GCACACG28450.019.7634611
GTATGCC29400.019.71156745
GCCAGTA42250.019.3560961
ATGCCGT29200.018.9015747
TCGTATG30200.018.61809743
ACCCGTA36050.018.37233232
ACGTCTG30850.018.225515
CGCGTAT14450.018.1485481
TGTGCGA37800.017.9781849
TGCGAGT38050.017.85949711
CGGGCGC20100.017.50455915
AAGGCGC39650.017.22550838
TAAGGCG41550.016.85315337
GCGAGTC41400.016.74745612
AGGCGCA41200.016.49391239
CACACGT34300.016.4931212
Produced by FastQC (version 0.11.2)