FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13118757
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150310.11457640384679736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG24900.030.47842846
CGTATGC25050.030.4328244
GTATGCC32500.023.66922845
CGCGCGT18850.023.6128581
TCGTATG32200.023.46087543
ATGCCGT34400.022.26197647
GCACACG35800.022.06514411
TCGGGCG24800.021.5610087
CACACGT39200.020.85530712
ACGTCTG41150.019.44796415
CGGGCAT26950.019.3325161
GGCGGGC24950.019.07939113
CTCGTAT38750.019.05020742
ATCGGGC28250.018.9317196
CGGGCGC25950.018.47708315
CGCGTAT15700.017.8014581
GCGGGCG26750.017.79547714
CGGCAAT39850.017.7498911
ACACGTC45250.017.60959413
CGCGCCT37250.017.32151
Produced by FastQC (version 0.11.2)