FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15034728
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT15950.026.1755831
CGTATGC27000.023.50832746
TATGCCG27950.022.83279248
CGCGCGT21900.021.1121461
TCGGGCG24700.019.8323867
CGGGCAT25950.019.8116471
CGGGCGC23250.019.73255715
GCACACG37800.019.52891311
GGCGGGC24500.018.72610513
TCGTATG32700.018.46093245
GCGGGCG25650.018.0207214
ATCGGGC29300.017.8996016
GTATGCC36900.017.29464147
CACACGT42000.017.24761412
GGGCGGC28500.016.8244369
ACGTCTG43350.016.78989615
CGGCAAT46000.016.5770071
ATGCCGT38200.016.43539249
CGCGGCT32300.016.237261
ACACGTC46050.015.95555413
Produced by FastQC (version 0.11.2)