FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a3_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a3_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11973307
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGT11300.021.9851821
CGGGCGC9350.018.81585315
GCACACG30650.018.45774811
CGCGTAT12500.018.2183881
CACACGT33800.017.55411312
ACGTCTG33150.017.37794115
CGCGCCT18200.017.2522621
GCGGGCG10200.017.24757814
ACACGTC34150.017.17217813
GGCGGGC10850.016.53258513
TCGGGCG11900.016.523587
CGGGCAT17050.016.1898041
CGCGGAT17050.015.9874321
CACGTCT40750.014.05201514
CGGGAAT32000.013.6939821
ATCGGGC15750.013.58210856
CGGCAAT28550.013.2942111
CGCGCAT9250.013.0557651
CGTCTGA46250.012.82868316
ATCGGGG13600.012.68214940
Produced by FastQC (version 0.11.2)