FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12929949
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG22800.022.54312148
CGCGCGT11800.021.9305421
CGTATGC24200.021.52414146
CGCGTAT12550.017.5956941
GTATGCC30000.017.24788947
ATGCCGT31000.017.13654549
TCGGGCG13100.017.1170227
CGGGCAT18650.016.8357581
TCGTATG30900.016.74552345
GCACACG33800.016.12468511
GCGGGCG12000.015.52220114
CGGGCGC12150.015.33056815
ACGTCTG36550.014.91112715
GGCGGGC13050.014.80227113
CACACGT38400.014.55240112
TATCTCG36850.014.50925341
CTCGTAT37350.014.22299444
ACACGTC39500.013.71050413
TGCCGTC39800.013.60757850
GCCGTCT43950.012.71511851
Produced by FastQC (version 0.11.2)