FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19284422
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC31250.023.51176844
TATGCCG32150.023.3903646
GCACACG42800.022.40537811
ACGTCTG44750.020.889815
CACACGT47700.019.8868312
CGGTAGC45900.019.84106633
AGTCACG47550.019.73285528
GTATGCC38850.019.71156345
GTGTGCG29350.019.04098
ACGGTAG51550.018.26856232
ACACGTC53050.017.75132413
CGGGCGC19400.017.06976515
ATGCCGT42800.016.84472547
GGTAGCA55700.016.78361934
TCGTATG43950.016.71769743
CACGGTA59100.016.16823831
GCCAGTA50700.015.7888151
GGCGGGC21850.015.62921813
CACGTCT60300.015.55975814
ACCCGTA37250.015.37277332
Produced by FastQC (version 0.11.2)