FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13734111
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC22350.024.07784746
TATGCCG25250.021.85878448
GCACACG27950.020.48742511
GTATGCC28350.019.22543547
GCCAGTA38750.019.0543141
GTGTGCG23100.018.368688
ACGTCTG31600.018.33912315
ATGCCGT30350.017.95845449
TCGTATG31150.017.93988445
AGTCACG33000.017.66627328
ACGAGTG35150.016.3897132
CACACGT35600.016.37568312
CTCGTAT35650.015.868731544
CGAGTGG36350.015.75374233
CGGGCGC13250.015.09970515
CACGAGT39600.014.89633431
GTCACGA39250.014.76528329
TATCTCG38250.014.6998941
GTGCGAG29100.014.2250610
ACACGTC41600.014.01385513
Produced by FastQC (version 0.11.2)