FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a1_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a1_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11058414
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA15050.025.90003235
CGTACGT17450.022.3387634
GTGTGCG25700.021.3429188
CCGTACG19000.020.51666633
CGTATGC15900.019.52610246
GCACACG22550.019.27446711
TACGTAA20400.018.93844436
TATGCCG16200.018.73862848
ACGTCTG23500.018.0542715
CGGGCGC22150.016.9744415
GTGCGAG32350.016.84802610
GGCGGGC23100.016.4263513
GTATGCC19850.016.33561747
ACGTAAT24350.016.008137
TGCGAGT34200.015.73464211
TGTGTGC36500.015.5949087
CACACGT27800.015.51049112
TCGTATG21400.015.15277345
GCCAGTA36600.014.9891341
TAGCCCG25150.014.95080534
Produced by FastQC (version 0.11.2)