FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10365029
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG21400.022.56724711
ACGTCTG23100.020.75692215
CACACGT24850.019.85061512
GTGTGCG18600.018.9175158
GCCAGTA36100.018.1604231
CGTTTCG24450.018.0596833
AGTCACG25250.017.6238628
GTCACGT25500.017.45099329
CACGTCT31550.015.74406714
ACACGTC32700.015.1906713
AGTAGTC43450.015.0876064
ACCCGTA25050.014.45960332
AGCACAC36300.014.44466810
GTGCGAG24500.014.22086110
CAGTAGT46900.014.0511813
CACGTTT33500.013.901531
CGGGCGC15750.013.79812615
CCAGTAG47500.013.7283532
TAGTCAT48150.013.3284456
CGCGTAT10450.013.207581
Produced by FastQC (version 0.11.2)