FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8241525
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150210.18225995795681016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19950.040.28789548
CGTATGC21500.037.38342346
GTATGCC24550.033.3010947
TCGTATG24750.032.47448745
GCACACG26950.031.61547911
ACGTCTG28200.029.96925415
ATGCCGT27100.029.53114349
AGTCACG28350.029.20336728
CACACGT29550.028.8335712
CTCGTAT27500.028.72528644
GTCACGT29150.028.04671129
CACGTGA31050.026.21986431
ACACGTC33600.025.15291813
CGCGCGT15650.025.1345251
TGCCGTC31900.025.08743350
TCACGTG32950.024.81203730
CACGTCT35500.023.70924814
GCCGTCT33600.023.20214351
AGCACAC38750.022.43329410
ACGTGAA37050.022.1599532
Produced by FastQC (version 0.11.2)