FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a0_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a0_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11641210
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC16600.033.87224633
TATGCCG19300.029.13464548
TCCGCAC20000.028.63115935
CGTATGC20300.027.86889646
CCGCACA20400.027.39338336
CGCGCGT15250.025.794461
ACGTCCG22150.025.38496432
CGCACAT22250.025.27109137
GTCCGCA23950.023.90910734
TCGGGCG20650.023.0530937
GTATGCC25350.022.86152547
CGCGTAT15100.022.8514861
GGCGGGC21300.021.05333113
GCACACG29550.020.54493711
TCGTATG27700.020.42360145
CGGGCGC21800.020.0958415
ATCGGGC23950.020.025616
ATGCCGT28800.019.64420949
CGGCAAT36250.019.4184061
GCGGGCG22900.019.28075814
Produced by FastQC (version 0.11.2)