FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a0_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a0_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9281608
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC19800.033.4506644
TATGCCG20150.032.1857246
GTATGCC24400.027.14468645
TCGTATG25500.026.24415443
GCACACG27000.025.55231511
TCACGTA26350.025.2678830
ATGCCGT26550.025.07699647
ACGTCTG28050.024.10402515
AGTCACG29000.023.55370928
GTCACGT29300.023.31254429
CTCGTAT29800.022.57324842
ACGTAGA30050.022.38629532
CACACGT31700.021.7642712
ACACGTC31950.021.48611613
CGCGCGT10500.021.0293031
TCGGGCG11700.020.639527
CGGGCGC11100.020.51102315
GGCGGGC11400.019.9716913
CACGTCT35100.019.65573314
CGTAGAG36400.019.42873833
Produced by FastQC (version 0.11.2)