FastQCFastQC Report
Mon 12 Mar 2018
HC22WBGX5_n01_a0_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHC22WBGX5_n01_a0_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10799350
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140670.1302578395921977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17550.024.96276348
CGCGCGT10150.024.4750181
CGTATGC19850.022.07045646
TCCGTAT28700.020.91382237
GTATGCC22350.019.91022747
GCACACG28450.019.76301811
TCGGGCG14050.019.151667
ACGTCTG29850.019.06758115
GGCGGGC12900.018.4509813
CGGGCGC12400.018.36023515
CCGTATC31100.017.96853438
TTCCGTA33200.017.66350636
ACACGTC32050.017.65126413
TCGTATG25550.017.55142845
CACACGT32050.017.5433912
CGCGCCT19400.017.4293381
ATGCCGT24950.017.2825449
CGCGTAT11200.017.2514881
CTCGTAT27650.017.09162944
GTTCCGT33900.016.78990735
Produced by FastQC (version 0.11.2)