FastQCFastQC Report
Wed 10 Dec 2014
LMN_Undetermined_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLMN_Undetermined_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105943316
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTGT3397800.044.6723182
AGGCTAT1195200.044.6520462
AGGCTCG1621500.044.57532
AGGCTAG538350.044.498352
AGGCTGA1320900.043.993912
TTACCTG228750.043.7075921
AGGCTCC1033150.043.6559222
GGCTATT472350.043.646413
AGGCTTA423000.043.3550222
GAGGCTG9167600.043.235011
GGCTAGG110500.043.0899053
GGCTGTA423250.042.9359933
GGCTGTG1105450.042.8158843
GGCTATC319500.042.63773
GAGGCTC6391750.042.4690131
GGCTCGG816350.042.3634573
AGGCTAC343950.042.328332
TTGCATG2737150.042.2596051
TGCATGT1047950.042.064532
GCTCGGG253500.041.8973164
Produced by FastQC (version 0.11.2)