FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l02n02 lacz21.3420000000e40d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l02n02 lacz21.3420000000e40d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16311321
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT57100.017.33800745
CTGACGC78500.016.36624545
TAGACCG30600.012.9411395
TCTGACG105900.012.23796445
CCGTGCG17900.011.3128179
TATACAC661750.010.93124545
AGACCGT37550.010.8455096
GTGCTAG19950.010.7143541
CCTTTGG53500.010.6402541
GTACTAG17850.010.5883021
TAGACAG29150.010.4973965
TACACTG61200.010.4779115
ATACACA498250.010.47215545
CTAGACC20850.010.4675964
GTCTAGT22500.010.4000661
GTATAGG50100.010.2395861
TACACAT350450.010.00927545
ACCGTGC24650.09.9492628
ATCTGAC132550.09.84534344
GTCTAGC18800.09.8138921
Produced by FastQC (version 0.11.2)