FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l02n02 lacz18.3420000000e3ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l02n02 lacz18.3420000000e3ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21200739
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG44750.08.1452455
GTATAAG115700.08.1288331
TCTAGGG16150.08.0805092
AGACCGT51250.07.9463366
GTATAGG66300.07.907291
GTATATA223900.07.8082231
CTAAGAC23750.07.7684343
TATAGGA56600.07.7120272
TATACTG81150.07.62476255
TACACTG75100.07.4300885
CCTACAC56050.07.3059893
CCTAGAC13350.07.24720243
GTATTAG68550.07.2210521
TAGGACC34000.07.213234
GTATACG42450.07.1555221
TCCTACA67150.07.002992
TCTATAC69650.06.9777573
CCGGGCT26200.06.95610214
TAGACAG36150.06.90870865
ATCCTAG27950.06.8426191
Produced by FastQC (version 0.11.2)