FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l02n02 lacz15.3420000000e3d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l02n02 lacz15.3420000000e3d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8875609
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTAGATCTCGGTGGTC369420.4162193264710061Illumina Single End PCR Primer 1 (95% over 22bp)
GTATAAGAGACAGCTGTCTCTTATACACATCTGACGCTGCCGACGAGTGTA103840.11699478875196057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG55950.035.02692443
CGGTGGT59800.032.0569644
GGTGGTC59250.031.48111745
CTCGGTG67150.029.15124942
TCTCGGT67750.028.92629241
AGATCTC75100.026.42470438
ATCTCGG75350.026.2474540
GTAGATC75750.025.96033336
GATCTCG78600.025.1907839
GTGTAGA80500.024.3168534
AGTGTAG85850.022.74905633
CTGCCGA86950.022.40950424
GCTGCCG89350.022.16011623
CCGACGA87400.022.0109427
CGCTGCC90100.021.90073622
CTGACGC91900.021.8878718
CGAGTGT87100.021.88009331
GACGAGT87000.021.87938129
TGACGCT91400.021.78605319
TGCCGAC88850.021.72770325
Produced by FastQC (version 0.11.2)