FastQCFastQC Report
Fri 24 Jul 2015
HBB8TADXX l02n02 gfp21.3420000000e41a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHBB8TADXX l02n02 gfp21.3420000000e41a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42849133
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGCT159950.017.17567645
CTGACGC211450.016.6741545
TCTGACG293450.012.38285545
TATACAC1586750.010.07767245
ATCTGAC362650.09.98896744
ATACACA1222300.09.69544845
TACACTG157450.09.4744115
CCGTGCG45700.09.3544619
CTCGGGG44550.09.19194830
TAGACCG79450.08.9773215
CGGGACT93750.08.92797610
GGACCGT29000.08.922396
TCGGGAC95250.08.8818669
GTGTAGG126100.08.7252561
AGACCGT92300.08.6050696
TATACTG147350.08.5968565
TTATACA2066950.08.53975445
TACACAT885800.08.53211645
ACCGTGC60400.08.5306078
CATCTGA430150.08.49470943
Produced by FastQC (version 0.11.2)